MYSM1 Knockout cell line (Hep G2)

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MYSM1 Knockout cell line (Hep G2) Order

MYSM1 Knockout cell line (Hep G2)
Catalog#
YKO-HT4027
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MYSM1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
MYSM1 Knockout cell line (Hep G2)
Species
Human
Gene Name
MYSM1
Gene ID
114803
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript MYSM1-202)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MYSM1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MYSM1 gene knockout MYSM1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
MYSM1 Knockout cell line (Hep G2)
MYSM1 Knockout cell line (Hep G2)
Catalog#
YKO-HT4027
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • MYSM1 Knockout cell line (U-2 OS)

    Organism: Human
    Gene Name: MYSM1
    Gene ID: 114803
    Size: 1*10^6
    Catalog#: YKO-HT13347
    Request a Quote More details

  • MYSM1 Knockout cell line (Jurkat)

    Organism: Human
    Gene Name: MYSM1
    Gene ID: 114803
    Size: 1*10^6
    Catalog#: YKO-HT11220
    Request a Quote More details

  • MYSM1 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: MYSM1
    Gene ID: 114803
    Size: 1*10^6
    Catalog#: YKO-HT8520
    Request a Quote More details

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